Experience true application freedom with a modular, future-proof automation platform designed to evolve with your laboratory. Featuring Push-to-Fill injection precision, an Open-Bed modular architecture, and multi-step chemical cleaning, the HT4000L redefines conventional LC autosamplers. Whether configured today as a cooled UHPLC system or tomorrow as a biocompatible, large-volume, or preparative autosampler, it protects your initial investment, maximizes equipment utilization, and fits any HPLC, UHPLC, IC, or LC-MS system brand.
Unlike conventional split-loop or flow-through-needle systems where continuous pressure and friction accelerate seal wear and narrow capillaries promote micro-bubble formation, the HT4000L takes a fundamentally different approach. By aspirating samples under atmospheric conditions and actively pushing them into the externally mounted injection valve and sample loop, the syringe and needle remain isolated from high-pressure mobile-phase flow during analysis. This eliminates pressure stress, prevents out gassing, removes the need for dedicated inline degassing lines, and ensures controlled, consistent loop filling for outstanding injection precision and long-term reproducibility.
Rethinking traditional stacked autosampler designs, the HT4000L features an open layout that keeps the sample deck, solvent panel, syringe compartment, and injection area fully visible and accessible from the front for faster, safer servicing. By expanding in bench depth rather than width, it delivers exceptionally high sample capacity while preserving valuable front-bench space. The open deck supports 2 to 40 ml vials, test tubes, tube blocks, and standard well plates (accommodating up to 176 × 2 ml vials, 96 × 4 ml vials, 54 × 20 ml vials, or three 96-well plates) to enable extended unattended operation and maximum autonomy for over 280 samples.
The isolated needle design enables effective needle cleaning without exposing the analytical column to aggressive wash solvents. By moving the needle to external rinse stations outside the analytical flow path, multi-step wash cycles can utilize up to two independent solvents. Aggressive solvents remove persistent residues from complex or sticky samples, followed by milder solvents to recondition the system. This guarantees minimal carryover and effective cleaning without altering the analytical method or compromising chromatographic integrity.
The HT4000L adapts seamlessly to conventional HPLC, fast HPLC, and UHPLC systems through selectable side-mounted injection valves matching your system pressure. A wide selection of loop volumes supports everything from routine analytical partial-loop applications (with fine-tuned volume control down to the nanoliter range) to fully automated large-volume injections up to 10 ml. Furthermore, inert PEEK flow paths ensure full compatibility with ion chromatography (IC) and biological applications, while an optional cooling module, dual-valve configurations (serving two independent systems simultaneously), and custom racks offer endless scalability.
Beyond standard liquid chromatography routines—such as dilution, derivatization, pH spiking, and internal standard addition—the HT4000L’s flexible architecture extends automated sample handling to non-conventional measurement systems. Integrated injection ports and dedicated transfer lines enable direct sample introduction into alternative detection setups. Controlled via the complementary HTA Autosampler Manager software (with optional CFR 21 Part 11 upgrade), the HT4000L transforms from a conventional autosampler into a multi-purpose sample preparation and introduction platform.
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HT4000L allows direct injection of the samples in your HPLC or LC-MS analyzer. Learn more how HT4000L is connected with your analyzer and about Chromatography Data System integration.
HT4000L method, sample list and setup editing will be performed by the free PC control (HTA Autosampler Manager), while all the routine operations can be managed by its dedicated keypad.
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